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2020 - 04 - 23

2020年4月20日訊 /生物谷BIOON /——無論是在雜貨店擦擦購物車后至少洗手20秒，還是盡可能呆在家里，我們都在適應(yīng)一種新常態(tài)，以限制感染SARS-CoV-2?，F(xiàn)在也是關(guān)注我們的免疫系統(tǒng)的好時(shí)機(jī)，如果我們暴露在疾病中，免疫系統(tǒng)會(huì)影響我們身體的反應(yīng)。它受到我們?nèi)粘Ａ?xí)慣的深刻影響。科羅拉多大學(xué)博爾德分校生態(tài)與進(jìn)化生物學(xué)的杰出教授、該系榮譽(yù)項(xiàng)目的聯(lián)合主任Barbara Demmig-Adams說：'小事情也能帶來改變。'Demmig-Adams研究的是植物抗氧化劑，它是我們飲食的重要組成部分，因?yàn)槿梭w自身無法產(chǎn)生這些營養(yǎng)物質(zhì)。她還教授一門關(guān)于生活方式醫(yī)學(xué)的本科課程，以便將植物對(duì)人類生活的重要性轉(zhuǎn)化為與學(xué)生相關(guān)的研究。圖片來源：https://cn.bing.com通過她自己的研究和教學(xué)，Demmig-Adams發(fā)現(xiàn)人類免疫系統(tǒng)的力量被現(xiàn)代生活的許多方面削弱了。我們坐得太多，吃能量豐富的食物，缺乏微量營養(yǎng)素，還要應(yīng)對(duì)長期的心理壓力和睡眠不足。但是一個(gè)人可以采取一些措施來改善他們的免疫健康。限制和管理壓力，獲得更多的休息睡眠，不僅可以提高免疫力，還可以幫助對(duì)抗焦慮、抑郁和其他許多疾病和失調(diào)。這些措施，加上均衡的飲食，也支持著人體的微生物群落，這些有益的微生物位于我們的外表面和內(nèi)表面。Demmig-Adams說，這些微生物不僅提供了抵御感染入侵者的第一道防線，而且還產(chǎn)生...

一次編輯數(shù)百種基因！遺傳學(xué)大牛George Church發(fā)布基因編輯新方法

2018 - 05 - 25

基因編輯技術(shù)幫助人們實(shí)現(xiàn)了“編輯生命”的愿望，但該技術(shù)自身存在的局限性也限制了其廣泛應(yīng)用，此前人們的研究熱情也多集中在編輯的效率、準(zhǔn)確性與安全性，但常規(guī)的基因編輯技術(shù)在一個(gè)反應(yīng)中僅能對(duì)一個(gè)或數(shù)個(gè)基因進(jìn)行操作，且通常會(huì)在基因組中留下額外的不需要的序列修改，在大型研究中，已有的基因編輯技術(shù)遠(yuǎn)遠(yuǎn)無法滿足大量不同基因的編輯需求。為解決上述問題，哈佛大學(xué)George Church教授領(lǐng)導(dǎo)的研究團(tuán)隊(duì)開發(fā)了一種基于CRISPR-Cas9的新型高通量基因編輯方法：guide+donor，該方法可以在單個(gè)釀酒酵母中同時(shí)精確改變數(shù)百個(gè)不同基因，編輯效率達(dá)到80％至100％，還能在生物體內(nèi)選擇特定行為細(xì)胞，進(jìn)行定向基因編輯和修復(fù)。guide+donor除幫助構(gòu)建大型文庫外，還能進(jìn)行基因功能分析，發(fā)現(xiàn)一些未知的基因突變和功能。5月21日，相關(guān)研究成果發(fā)表在著名學(xué)術(shù)期刊Nature Biotechnology上，文章題為“High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast”。George Church教授人類的基因變異通常是點(diǎn)突變導(dǎo)致，為在不干擾其他潛在變異的情況下重構(gòu)釀酒酵母中的某些變異，CRISPR-Cas9技術(shù)借助sgRNA精確靶向D...

揭秘細(xì)菌挑食之謎

2019 - 03 - 22

給你一個(gè)冰淇淋和一塊蛋糕，你先吃哪個(gè)？一塊面包和一個(gè)牛油果呢？兩個(gè)一塊吃嗎？1941 年微生物學(xué)家 Jacques Monod 給細(xì)菌提出了同樣的問題。他給大腸桿菌同時(shí)提供兩種食物（碳源），結(jié)果看到了兩類不同的現(xiàn)象：對(duì)某一些食物組合（比麥芽糖和蘋果酸），細(xì)菌兩種同時(shí)吃，而對(duì)另一些組合（比如葡萄糖和乳糖），細(xì)菌要先吃完一種（葡萄糖），然后再會(huì)去吃另一種（乳糖）。隨后 Monod 和 François Jacob 一道，找出了細(xì)菌在有葡萄糖和乳糖的情況下只吃葡萄糖的分子機(jī)制—乳糖操縱子：在這兩種糖都存在的時(shí)候，細(xì)胞不會(huì)去表達(dá)運(yùn)輸和消化乳糖的相關(guān)基因，并獲得了 1965 年的諾貝爾生理學(xué)獎(jiǎng)。可是為什么細(xì)菌要挑食呢？為什么對(duì)于有些食物的組合它們又不挑呢？北京大學(xué)湯超教授團(tuán)隊(duì)發(fā)現(xiàn)細(xì)菌的這一套進(jìn)食策略能夠使它們最優(yōu)化的生長。如果哪個(gè)菌種想任性一把，同時(shí)吃葡萄糖和乳糖，或者對(duì)麥芽糖和蘋果酸挑食，那么它們的代價(jià)就是生長速率的降低，進(jìn)而面臨被淘汰的危險(xiǎn)。結(jié)合代謝網(wǎng)絡(luò)的拓?fù)涮卣骱图?xì)胞內(nèi)蛋白資源的優(yōu)化分配，研究團(tuán)隊(duì)的理論模型定量的解釋了細(xì)菌面對(duì)多種碳源的進(jìn)食策略。在兩類碳源同時(shí)被細(xì)菌消耗的情況下，模型預(yù)測(cè)并用實(shí)驗(yàn)驗(yàn)證了細(xì)菌內(nèi)不同氨基酸上的碳原子來自兩種碳源的比例。這個(gè)例子進(jìn)一步說明了在看似復(fù)雜的生命現(xiàn)象中，存在簡潔的原理與定量的規(guī)律。相關(guān)文章發(fā)表在《自然 - 通訊》雜志上。北京大學(xué)定量生物學(xué)中...

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產(chǎn)品名稱： EVOS? Vessel Holder, universal

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder has a 25.4 mm opening and accommodates most vessel types. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samp...

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產(chǎn)品名稱： EVOS? Vessel Holder, Greiner T-75 flask

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Greiner T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ves...

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產(chǎn)品名稱： EVOS? Vessel Holder, Hemocytometer

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates a hemocytometer. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel hol...

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產(chǎn)品名稱： EVOS? Vessel Holder, Nunc? T-75 flask

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Nunc® T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ...

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產(chǎn)品名稱： EVOS? Vessel Holder, two 25-cm T-flasks

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates two 25 cm T-flasks. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel ...

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產(chǎn)品名稱： EVOS? Vessel Holder, one 100-mm Petri dish

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one 100 mm Petri dish. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vess...

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產(chǎn)品名稱： EVOS? Vessel Holder, two 60-mm Petri dishes

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時(shí)間： 2019 - 04 - 15

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates two 60 mm Petri dishes. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ves...

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產(chǎn)品名稱： EVOS? Vessel Holder, four 35-mm Petri dishes

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時(shí)間： 2019 - 04 - 12

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates four 35 mm Petri dishes. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ve...

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產(chǎn)品名稱： EVOS? Vessel Holder, two 25 x 75 mm slides

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時(shí)間： 2019 - 04 - 12

描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates two 25 x 75 mm slides. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vess...

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產(chǎn)品名稱： EVOS? 4X Objective, fluorite, LWD, phase-contrast

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時(shí)間： 2019 - 04 - 12

描述This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies nee...

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產(chǎn)品名稱： EVOS? Light Cube, Cy?5

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時(shí)間： 2019 - 04 - 12

描述All EVOS® fluorescence imaging systems and the Countess® II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The Cy®5 light cube is ideal for use with Alexa Fluor&...

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產(chǎn)品名稱： EVOS? Light Cube, RFP

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時(shí)間： 2019 - 04 - 12

描述All EVOS® fluorescence imaging systems and the Countess® II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS® light cubes are user-changeable and automatically recognized by the system. The RFP light cube is ideal for use with Alexa Fluor® ...

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